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Difco-BRL) with or without 50 µg/ml kanamycin (LB kan50 ), incubated at 37 °C overnight, then manually scored for colony forming units (CFU), with the percent of kanamycin resistant (kan r ) determined by dividing the number of CFU on the LB kan50 by the number of CFU on LB, and multiplying by 100

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Therefore, it is essential that you can receive maximum benefits from the shots